Recognition of cytoplasmic yeast antigens ofCryptococcus neoformansvar.neoformansandCryptococcus neoformansvar.gattiiby immune human sera

Identification and purification of specific Penicillium marneffei antigens and their recognition by human immune sera.

Disseminated infection with the dimorphic pathogenic fungus Penicillium marneffei is increasingly seen among patients with AIDS in southeast Asian countries. Previous studies have demonstrated the presence of humoral immune responses to this fungus in patient sera; we have confirmed this work using sera from P. marneffei-infected patients (n = 21) to develop Western blots of P. marneffei cytopl...

Leukocyte antibodies in human sera and in immune rabbit sera.

Recognition of Histoplasma capsulatum yeast-cell antigens by human lymphocytes and human T-cell clones.

We have prepared a detergent extract from the cell wall and cell membrane of Histoplasma capsulatum yeast cells that is recognized by T cells from mice immunized with viable organisms or with the extract. A 62-kd antigen from this extract has been isolated and has been shown to be antigenic and to confer protective immunity in mice. In this study, we examined the in vitro proliferative response...

Antigenic Patterns in Human Sera: Race-Specific Antigens *

The information available relating to the several phases of the problem of specificity in serological reactions has been thoroughly canvassed by Landsteiner,3 who has prepared an admirable critical statement of the results accumulated from the days of Uhlenhuth and of Nuttall down to the year 1936. From his text it is evident that, by various technics, it is readily possible to distinguish sero...

Identification of antigens of pathogenic free-living amoebae by protein immunoblotting with rabbit immune and human sera.

Prominent antigens of pathogenic and nonpathogenic free-living amoebae were identified by using polyclonal rabbit immune sera in immunoblot assays. The intent was to determine if prominent epitopes identified with rabbit immune sera could also be recognized by human sera. With rabbit sera, the development of immunoreactive bands was restricted to molecular masses of greater than 18.5 kDa for Na...